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Immunohistochemical staining of human fallopian tube shows strong positivity in a subset of glandular cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in a subset of glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 45 cell types
RNA: detected in 35 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1800
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Staining in subset of neurons throughout the brain, strongest expression and immunoreactivity in hippocampus and cerebellum.
WESTERN BLOT
Antibody HPA019055
Antibody HPA028849
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402864)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402864)
Target mass (kDa)
33.8, 33.2, 29.4, 27.6, 20.7
33.9, 33.8, 33.2, 29.4, 27.6, 20.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019055
Antibody HPA028849
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
