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CTC-360G5.8

ANTIBODY INFORMATION
 
 

Antibody HPA052730

  

Antibody HPA056957

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052730   HPA056957  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 SARS2 - ENSG00000104835 [100%]
   SARS2 - ENSG00000104835 [100%]
  

Released in version

 12   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA052730

  

Antibody HPA056957

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052730

  

Antibody HPA056957

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern in tubular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 63 cell types		   RNA: detected in 2 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:500  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052730

  

Antibody HPA056957

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 65.6, 58.3, 58.2, 58, 36.4   65.6, 58.3, 58.2  

Antibody dilution

 1:110   1:570  

PROTEIN ARRAY
 
 

Antibody HPA052730

  

Antibody HPA056957

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:850   1:6850  

ANTIGEN INFORMATION
 
 

Antibody HPA052730

  

Antibody HPA056957

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   74  

Antigen sequence

 
QIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRA
ETNTGQEPRGLYRVHHFTKVEMFGVTGPGL
  
RSLEEEKAAVTEAVRALLANQDSGEVQQDPKYQGLRARGREIRKELVHLY
PREAQLEEQFYLQALKLPNQTHPD
 

Matching transcripts

 CTC-360G5.8-001 - ENSP00000472465 [100%]
  CTC-360G5.8-001 - ENSP00000472465 [100%]
 

Other gene match

 SARS2 - ENSG00000104835 [100%]
   SARS2 - ENSG00000104835 [100%]
  
ANTIGEN VIEW
CTC-360G5.8-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.