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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:76
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038748
Antibody HPA038752
Antibody HPA042595
Antibody HPA043105
Antibody HPA043692
Antibody HPA044023
Antibody HPA046971
Antibody HPA058050
Standard validation
Uncertain
Uncertain
Uncertain
Uncertain
Uncertain
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human uterus, post-menopause shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong dot like positivity in subset of cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 60 cell types
RNA: detected in 37 tissues Protein: detected in 20 cell types
RNA: detected in 37 tissues Protein: detected in 12 cell types
RNA: detected in 37 tissues Protein: detected in 8 cell types
RNA: detected in 37 tissues Protein: detected in 5 cell types
RNA: detected in 37 tissues Protein: detected in 27 cell types
RNA: detected in 37 tissues Protein: detected in 13 cell types
RNA: detected in 37 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:50
1:1300
1:300
1:1700
1:500
1:35
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038748
Antibody HPA038752
Antibody HPA042595
Antibody HPA043105
Antibody HPA043692
Antibody HPA044023
Antibody HPA046971
Antibody HPA058050
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
