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CFAP206

ANTIBODY INFORMATION
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044891   HPA072261  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044891

  

Antibody HPA072261

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.  

Antibody dilution

   1:95  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.    

Expression

 RNA: detected in 5 tissues
Protein: detected in 26 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 71.2   71.2  

Antibody dilution

 1:250   1:320  

PROTEIN ARRAY
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3800  

ANTIGEN INFORMATION
 
 

Antibody HPA044891

  

Antibody HPA072261

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 135   92  

Antigen sequence

 
ELGTFLTLSKKDKERQLKELTMIVTGIRLFNRDCGKGGEGIDDLPAVLHV
AIPATMQHIDYQLETARSQVYRYTAILEKAANDPLMRAELQPYMLKEALY
NIRQYEVFLQIILSDIITGAQEVEMMTKQLGAHLE
  
GVLSNLFTHIQPFLGAHELYFPERVMQCHLNGATVKTDVCRMKEHMEDRV
NVADFRKLEWLFPETTANFDKLLIQYRGFCAYTFAATDGLLL
 

Matching transcripts

 CFAP206-001 - ENSP00000358575 [100%]
  CFAP206-001 - ENSP00000358575 [100%]
 

Other gene match

    
ANTIGEN VIEW
CFAP206-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.