Fields »
ABOUT
HELP
BLOG
PPP4R3B

ANTIBODY INFORMATION
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Kazusa DNA Research Institute
  

Product name

 HPA001233   GX0690  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 1   4  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA001233

  

Antibody CAB017185

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.  

Antibody dilution

 1:44   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.   Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 97.5, 93.7, 87.4   97.5, 93.7, 87.4  

Antibody dilution

 1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001233

  

Antibody CAB017185

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 65    

Antigen sequence

 
GQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFN
EDEEEEGKAVMPPLE
   

Matching transcripts

 PPP4R3B-001 - ENSP00000478677 [94%]
PPP4R3B-002 - ENSP00000483228 [94%]
PPP4R3B-003 - ENSP00000484116 [94%]
   

Other gene match

    
ANTIGEN VIEW
PPP4R3B-001
PPP4R3B-002
PPP4R3B-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA001233

  

Antibody CAB017185

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.