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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 34 tissues Protein: detected in 70 cell types
RNA: detected in 34 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:18
1:50
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030268
Antibody CAB004423
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409847)
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
13.1, 12.3, 10.7
13.1, 12.3, 10.7, 7.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA030268
Antibody CAB004423
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.