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AC253572.1

ANTIBODY INFORMATION
 
 

Antibody HPA046392

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046392  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 NOTCH2 - ENSG00000134250 [100%]
NOTCH2NL - ENSG00000264343 [100%]
NBPF14 - ENSG00000270629 [100%]
NBPF19 - ENSG00000271383 [100%]
  

Released in version

 11  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA046392

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046392

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in subset of glandular cells.  

Expression

 RNA: detected in 26 tissues
Protein: detected in 6 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:225  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046392

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 265.4, 92.6, 27.3, 25.9, 25.8, 25.5, 24.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA046392

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA046392

  

Antigen

 Recombinant protein fragment  

Length (aa)

 36  

Antigen sequence

 
TCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSR
 

Matching transcripts

 AC253572.1-201 - ENSP00000485259 [97%]
 

Other gene match

 NOTCH2 - ENSG00000134250 [100%]
NOTCH2NL - ENSG00000264343 [100%]
NBPF14 - ENSG00000270629 [100%]
NBPF19 - ENSG00000271383 [100%]
  
ANTIGEN VIEW
AC253572.1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.