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FKBP4

ANTIBODY INFORMATION
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Enzo Life Sciences
  

Product name

 HPA006148   HPA062857   ADI-SRA-1400  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   13   4  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated
     Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA062857.		  
Validated

Antibody staining overlaps with antibody HPA006148.		    
 

Standard validation

 Supported   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:125   1:147    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.      
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.     Immunohistochemical staining of human testis shows nuclear positivity in cells of the seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		     RNA: detected in 37 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:1250     1:1700  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 51.8   51.8   51.8, 9.5, 5.5, 2.5  

Antibody dilution

 1:250   1:490   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA006148

  

Antibody HPA062857

  

Antibody CAB017441

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 122   89    

Antigen sequence

 
IATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLT
EEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIG
EGENLDLPYGLERAIQRMEKGE
  
DFELARADFQKVLQLYPNNKAAKTQLAVCQQRIRRQLAREKKLYANMFER
LAEEENKAKAEASSGDHPTDTEMKEEQKSNTAGSQSQVE
  

											 
																				

																		

										
											
Matching transcripts

												
																					FKBP4-001 - ENSP00000001008 [100%] 

											 
																						FKBP4-001 - ENSP00000001008 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FKBP4-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006148

												
										 
																				
											
											
											
Antibody HPA062857

												
										 
																				
											
											
											
Antibody CAB017441

												
										 
																		

																	

										
											
1.

												
																						Cancer cell response to anthracyclines effects: mysteries of the hidden proteins associated with these drugs

	Tyleckova J et al

	Int J Mol Sci 2012;13(12):15536-64

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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