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ABCB5

ANTIBODY INFORMATION
 
 

Antibody HPA026975

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026975  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

 4  

VALIDATION SUMMARY
 
 

Antibody HPA026975

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026975

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human prostate shows strong granular positivity in glandular cells.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 48 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:160  

Literature conformity

 Not consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026975

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 138.6, 89.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA026975

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:100000  

ANTIGEN INFORMATION
 
 

Antibody HPA026975

  

Antigen

 Recombinant protein fragment  

Length (aa)

 90  

Antigen sequence

 
DLIVTLKDGMLAEKGAHAELMAKRGLYYSLVMSQDIKKADEQMESMTYST
ERKTNSLPLHSVKSIKSDFIDKAEESTQSKEISLPEVSLL
 

Matching transcripts

 ABCB5-001 - ENSP00000258738 [100%]
ABCB5-004 - ENSP00000384881 [100%]
 

Other gene match

  
ANTIGEN VIEW
ABCB5-001
ABCB5-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA026975

  

1.

 The helicase HAGE expressed by malignant melanoma-initiating cells is required for tumor cell proliferation in vivo
Linley AJ et al
J Biol Chem 2012;287(17):13633-43
  

2.

 ABCB5 expression and cancer stem cell hypothesis in oral squamous cell carcinoma
Grimm M et al
Eur J Cancer 2012;48(17):3186-97
  

3.

 The helicase HAGE prevents interferon-α-induced PML expression in ABCB5+ malignant melanoma-initiating cells by promoting the expression of SOCS1
Mathieu MG et al
Cell Death Dis 2014;5:e1061
  

4.

 Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Mathan JJ et al
Stem Cell Res Ther 2016;7(1):81
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.