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CDC27

ANTIBODY INFORMATION
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA028129   HPA052399   sc-9972  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 6   12   4  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA052399.		  
Validated

Antibody staining overlaps with antibody HPA028129.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:50   1:50   1:13  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.     Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		     RNA: detected in 37 tissues
Protein: detected in 80 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:10     1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 92.6, 91.9, 91.7   92.6, 91.9, 91.7, 25.9   92.6, 91.9, 91.7, 25.9, 18.8  

Antibody dilution

 1:250   1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA028129

  

Antibody HPA052399

  

Antibody CAB016315

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 81   92    

Antigen sequence

 
YKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDP
KGANNQIKEAIDKRYLPDDEEPITQEEQIMG
  
AIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRL
LKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKS
  

											 
																				

																		

										
											
Matching transcripts

												
																					CDC27-001 - ENSP00000066544 [100%] 
CDC27-002 - ENSP00000434614 [100%] 
CDC27-005 - ENSP00000437339 [100%] 

											 
																						CDC27-001 - ENSP00000066544 [100%] 
CDC27-002 - ENSP00000434614 [100%] 
CDC27-005 - ENSP00000437339 [100%] 
CDC27-012 - ENSP00000460454 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDC27-001
CDC27-002
CDC27-005
CDC27-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA028129

												
										 
																				
											
											
											
Antibody HPA052399

												
										 
																				
											
											
											
Antibody CAB016315

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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