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HCCS

ANTIBODY INFORMATION
 
 

Antibody HPA002946

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002946  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 2  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA002946

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002946

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		  

 

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in mitochondria and GFP expression in mitochondria.  

Antibody dilution

 1:61  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.  

Antibody dilution

 1:61  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002946

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:35  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002946

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.6  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA002946

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA002946

  

Antigen

 Recombinant protein fragment  

Length (aa)

 144  

Antigen sequence

 
YVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRA
DSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAW
KEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYEL
 

Matching transcripts

 HCCS-001 - ENSP00000326579 [100%]
HCCS-002 - ENSP00000370139 [100%]
HCCS-003 - ENSP00000370140 [100%]
 

Other gene match

  
ANTIGEN VIEW
HCCS-001
HCCS-002
HCCS-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA002946

  

1.

 Global profiling of co- and post-translationally N-myristoylated proteomes in human cells
Thinon E et al
Nat Commun 2014;5:4919
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.