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Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Antibody dilution
1:40
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA006770
Antibody HPA035623
Standard validation
Supported
Figure description
Immunohistochemical staining of human corpus, uterine shows moderate nuclear positivity in endometrial stromal cells.
Expression
RNA: detected in 12 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006770
Antibody HPA035623
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417250)
Target mass (kDa)
34.5, 31
34.5, 31
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA006770
Antibody HPA035623
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
