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Antibody staining overlaps with antibody HPA051960.
Validated
Antibody staining overlaps with antibody HPA045074.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
Antibody dilution
1:61
1:36
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA045074
Antibody HPA051960
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045074
Antibody HPA051960
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421772)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421772)
Target mass (kDa)
39.2, 23.7
39.2, 23.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA045074
Antibody HPA051960
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
