Fields »
ABOUT
HELP
BLOG
ELAC2

ANTIBODY INFORMATION
 
 

Antibody HPA019535

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019535  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA019535

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019535

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:49  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:50  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019535

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human oral mucosa shows strong nucleolar positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:60  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019535

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 92.2, 90, 87.6, 70.1, 31.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA019535

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019535

  

Antigen

 Recombinant protein fragment  

Length (aa)

 108  

Antigen sequence

 
WMERFGPDTQHLVLNENCASVHNLRSHKIQTQLNLIHPDIFPLLTSFRCK
KEGPTLSVPMVQGECLLKYQLRPRREWQRDAIITCNPEEFIVEALQLPNF
QQSVQEYR
 

Matching transcripts

 ELAC2-001 - ENSP00000337445 [100%]
ELAC2-002 - ENSP00000379291 [100%]
ELAC2-007 - ENSP00000406192 [100%]
ELAC2-012 - ENSP00000405223 [100%]
ELAC2-013 - ENSP00000463740 [100%]
 

Other gene match

  
ANTIGEN VIEW
ELAC2-001
ELAC2-002
ELAC2-007
ELAC2-012
ELAC2-013
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.