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Immunohistochemical staining of human pancreas shows strong memranous positivity in intercalated ducts.
Immunohistochemical staining of human pancreas shows strong positivity in luminal membranes of pancreatic ducts.
Immunohistochemical staining of human gall bladder shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 28 tissues Protein: detected in 27 cell types
RNA: detected in 28 tissues Protein: detected in 36 cell types
RNA: detected in 28 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:8
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004922
Antibody HPA031053
Antibody CAB011525
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
97.2, 96.2, 94.6, 93.7
97.2, 96.2, 94.6, 93.7
97.2, 96.2, 94.6, 93.7, 10.5, 6.1, 4.1, 1.4
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA004922
Antibody HPA031053
Antibody CAB011525
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
