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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
Antibody dilution
1:30
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA009178
Antibody CAB009751
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra and weak to moderate in reaction center cells.
Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in cells of the germinal center corona.
Expression
RNA: detected in 33 tissues Protein: detected in 40 cell types
RNA: detected in 33 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA009178
Antibody CAB009751
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421857)
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.1, 26, 14
26.1, 26, 14
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA009178
Antibody CAB009751
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
