We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:9
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Antibody dilution
1:9
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030775
Antibody CAB034143
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells.
Expression
RNA: detected in 11 tissues Protein: detected in 24 cell types
RNA: detected in 11 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:30
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA030775
Antibody CAB034143
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.2, 46.7
48.2, 46.7, 13.4, 6.7, 6.1, 4.2, 0.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA030775
Antibody CAB034143
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
