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Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in lymphoid cells outside reaction centra.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in subset of non-germinal center cells.
Expression
RNA: detected in 36 tissues Protein: detected in 50 cell types
RNA: detected in 36 tissues Protein: detected in 32 cell types
RNA: detected in 36 tissues Protein: detected in 6 cell types
RNA: detected in 36 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH9
HIER pH6
Antibody dilution
1:15
1:75
1:25
1:125
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004252
Antibody HPA004472
Antibody CAB000011
Antibody CAB068180
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400209)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.1
51.1
51.1, 16.5, 4.8
51.1, 16.5, 4.8
Antibody dilution
1:250
1:250
1:500
1:1000
PROTEIN ARRAY
Antibody HPA004252
Antibody HPA004472
Antibody CAB000011
Antibody CAB068180
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
