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Immunofluorescent staining of human cell line SiHa shows localization to nucleus & centrosome.
Antibody dilution
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038245
Antibody HPA038617
Antibody HPA064831
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:175
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038245
Antibody HPA038617
Antibody HPA064831
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411776)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411776)
Target mass (kDa)
23.9
20
23.9, 20
Antibody dilution
1:250
1:250
1:80
PROTEIN ARRAY
Antibody HPA038245
Antibody HPA038617
Antibody HPA064831
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
