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CENPQ

ANTIBODY INFORMATION
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029043   HPA046634   HPA054965  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 13   12   12  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry

  Approved    
Western blot

Uncertain   Supported   Uncertain  
Protein array

Approved   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.     Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.  

Antibody dilution

 1:81     1:99  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Standard validation

   Approved    

     

Figure description

   Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.    

Expression

   RNA: detected in 36 tissues
Protein: detected in 25 cell types		    

Retrieval

   HIER pH6    

Antibody dilution

   1:280    

Literature conformity

   Consistent with gene/protein characterization data.    

RNA consistency

   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 30.6   30.6   30.6  

Antibody dilution

 1:250   1:430   1:500  

PROTEIN ARRAY
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3450   1:3950  

ANTIGEN INFORMATION
 
 

Antibody HPA029043

  

Antibody HPA046634

  

Antibody HPA054965

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   88   72  

Antigen sequence

 
SGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSS
EGQTKHTNLKHGKTAASKRKTWQPLSKSTRDHLQTMME
  
SGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSS
EGQTKHTNLKHGKTAASKRKTWQPLSKSTRDHLQTMME
  
VIMTILSNSIKEKEEIQYHLNFLKKRLLQQCETLKVPPKKMEDLTNVSSL
LNMERARDKANEEGLALLQEEI
 

Matching transcripts

 CENPQ-001 - ENSP00000337289 [100%]
  CENPQ-001 - ENSP00000337289 [100%]
  CENPQ-001 - ENSP00000337289 [100%]
 

Other gene match

      
ANTIGEN VIEW
CENPQ-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.