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SLC4A7

ANTIBODY INFORMATION
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA035857   AB3296P  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 8   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035857

  

Antibody CAB022494

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & focal adhesion sites.  

Antibody dilution

   1:23  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic membranous positivity in glandular cells.   Immunohistochemical staining of human duodenum shows distinct membranous and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 6 cell types		   RNA: detected in 36 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:90  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 140.8, 139.3, 136, 135.3, 134.9, 134.6   140.8, 139.3, 136, 135.3, 134.9, 134.6, 127.4, 123.4, 123.1, 118.5, 89.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA035857

  

Antibody CAB022494

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 80    

Antigen sequence

 
KHSDPHLLERNGEGLSASRHSLRTGLSASNLSLRGESPLSLLLGHLLPSS
RAGTPAGSRCTTPVPTPQNSPPSSPSISRL
   

Matching transcripts

 SLC4A7-001 - ENSP00000295736 [100%]
SLC4A7-014 - ENSP00000390394 [100%]
SLC4A7-006 - ENSP00000414797 [86%]
SLC4A7-010 - ENSP00000406804 [86%]
SLC4A7-011 - ENSP00000406605 [86%]
SLC4A7-201 - ENSP00000401949 [86%]
   

Other gene match

    
ANTIGEN VIEW
SLC4A7-001
SLC4A7-006
SLC4A7-010
SLC4A7-011
SLC4A7-014
SLC4A7-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.