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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.
Antibody dilution
1:161
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028911
Antibody CAB037020
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
Expression
RNA: detected in 15 tissues Protein: detected in 69 cell types
RNA: detected in 15 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:1000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028911
Antibody CAB037020
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411602)
Target mass (kDa)
27.8
27.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA028911
Antibody CAB037020
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
