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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
Antibody dilution
1:400
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019849
Antibody CAB017712
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows strong apical membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 26 tissues Protein: detected in 34 cell types
RNA: detected in 26 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019849
Antibody CAB017712
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418170)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.6
24.6, 15.1, 9.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA019849
Antibody CAB017712
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
