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Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Antibody dilution
1:40
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028961
Antibody HPA042309
Standard validation
Approved
Figure description
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 36 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
Antibody dilution
1:350
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028961
Antibody HPA042309
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403212)
Target mass (kDa)
40.5
40.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA028961
Antibody HPA042309
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
