CAPG - Antibodies - The Human Protein Atlas

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CAPG

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018843

HPA019080

HPA019092

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:54

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus.

Antibody dilution

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.

Immunohistochemical staining of human lung shows strong cytoplasmic and nuclear positivity in macrophages.

Expression

RNA: detected in 37 tissues
Protein: detected in 57 cell types

RNA: detected in 37 tissues
Protein: detected in 17 cell types

RNA: detected in 37 tissues
Protein: detected in 23 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

1:250

1:200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

38.5, 36.9, 11.6

38.5, 36.9, 27.8, 24.1

38.5, 36.9, 27.8

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA018843

Antibody HPA019080

Antibody HPA019092

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

SSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFIS
RMQYAPNTQVEILPQGRESPIFKQF

PFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSH
LHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLF

ERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNP
EEDLTADKANAQAAALYKVSDATGQMNLTKVAD

Matching transcripts

CAPG-001 - ENSP00000263867 [99%]
CAPG-002 - ENSP00000386965 [99%]
CAPG-003 - ENSP00000386315 [99%]
CAPG-004 - ENSP00000387063 [99%]
CAPG-016 - ENSP00000397381 [99%]

CAPG-001 - ENSP00000263867 [100%]
CAPG-002 - ENSP00000386965 [100%]
CAPG-003 - ENSP00000386315 [100%]
CAPG-004 - ENSP00000387063 [100%]
CAPG-005 - ENSP00000391923 [100%]
CAPG-008 - ENSP00000398232 [100%]
CAPG-011 - ENSP00000403330 [100%]
CAPG-012 - ENSP00000386596 [100%]

CAPG-001 - ENSP00000263867 [100%]
CAPG-002 - ENSP00000386965 [100%]
CAPG-003 - ENSP00000386315 [100%]
CAPG-004 - ENSP00000387063 [82%]
CAPG-005 - ENSP00000391923 [82%]
CAPG-008 - ENSP00000398232 [82%]
CAPG-011 - ENSP00000403330 [82%]

Other gene match

ANTIGEN VIEW

CAPG-001	CAPG-002	CAPG-003	CAPG-004	CAPG-005	CAPG-008	CAPG-011	CAPG-012	CAPG-016

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.