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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA005561
Standard validation
Supported
Figure description
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor layer and outer plexiform layer.
Expression
RNA: detected in 2 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
Antibody dilution
1:14000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
Standard validation
Approved
Figure description
Axonal/Synaptic positivity was observed in most areas of the brain. Neuronal cell bodies were also positively stained, with predominant expression in midbrain.
WESTERN BLOT
Antibody HPA005561
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
23.5, 22.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA005561
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
