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PLEKHA5

ANTIBODY INFORMATION
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035923   HPA035924   HPA041455  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 7   7   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

     1:78  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:800   1:40    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 146.9, 134.3, 127.5, 125.5, 99.7, 45.2   146.9, 134.3, 127.5, 125.5, 111.9, 99.7   146.9, 134.3, 127.5, 125.5, 111.9, 99.7  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:12000  

ANTIGEN INFORMATION
 
 

Antibody HPA035923

  

Antibody HPA035924

  

Antibody HPA041455

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   101   93  

Antigen sequence

 
PNGVNSVEMMDKERNKDKMPEDVTFSPQDETQTANHKPEEHPEENTKNSV
DEQEETVISYESTPEVSRGNQTMAVKSLSPSPESSASPVPSTQP
  
PRHSTLSSPKTMVNISDQTMHSIPTSPSHGSIAAYQGYSPQRTYRSEVSS
PIQRGDVTIDRRHRAHHPKHVYVPDRRSVPAGLTLQSVSPQSLQGKTLSQ
D
  
NSVKLNSLPSEYESGSACPAQTVHYRPINLSSSENKIVNVSLADLRGGNR
PNTGPLYTEADRVIQRTNSMQQLEQWIKIQKGRGHEEETRGVI
 

Matching transcripts

 PLEKHA5-001 - ENSP00000439673 [100%]
PLEKHA5-002 - ENSP00000299275 [100%]
PLEKHA5-004 - ENSP00000404296 [100%]
PLEKHA5-006 - ENSP00000443553 [100%]
PLEKHA5-201 - ENSP00000400411 [100%]
PLEKHA5-202 - ENSP00000440611 [100%]
  PLEKHA5-002 - ENSP00000299275 [100%]
PLEKHA5-005 - ENSP00000440371 [100%]
PLEKHA5-202 - ENSP00000440611 [100%]
PLEKHA5-001 - ENSP00000439673 [96%]
PLEKHA5-004 - ENSP00000404296 [96%]
PLEKHA5-201 - ENSP00000400411 [96%]
  PLEKHA5-001 - ENSP00000439673 [100%]
PLEKHA5-002 - ENSP00000299275 [100%]
PLEKHA5-004 - ENSP00000404296 [100%]
PLEKHA5-005 - ENSP00000440371 [100%]
PLEKHA5-201 - ENSP00000400411 [100%]
PLEKHA5-202 - ENSP00000440611 [100%]
 

Other gene match

      
ANTIGEN VIEW
PLEKHA5-001
PLEKHA5-002
PLEKHA5-004
PLEKHA5-005
PLEKHA5-006
PLEKHA5-201
PLEKHA5-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.