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SYNE2

ANTIBODY INFORMATION
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003435   HPA050204  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 2   12  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA003435

  

Antibody HPA050204

  
Immunocytochemistry

Uncertain   Supported  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Standard validation

 Uncertain   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nuclear membrane.   Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane & intermediate filaments.  

Antibody dilution

 1:105   1:43  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear membrane positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong nuclear membranous and cytoplasmic membrane positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 798.9, 796.4, 788.4, 787.7   798.9, 796.4, 788.4, 787.7, 408.7, 377.2  

Antibody dilution

 1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA003435

  

Antibody HPA050204

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   138  

Antigen sequence

 
NVLNDAYENLTRYKEAVTRAVESITSLEAIIIPYRVDVGNPEESLEMPLR
KQEELESTVARIQDLTEKLGMISSPEAKLQLQYTLQELVSKNSAMKEAFK
AQETEAE
  
GTTPPIEADTLDSSDAQGGLEPRVEKTRPEPTEVLHACKTQVAELELWLQ
QANVAVEPETLNADMQQVLEQQLVGCQAMLTEIEHKVAFLLETCKDQGLG
DNGATQHEAEALSLKLKTVKCNLEKVQMMLQEKHSEDQ
 

Matching transcripts

 SYNE2-001 - ENSP00000341781 [99%]
SYNE2-002 - ENSP00000452570 [99%]
SYNE2-201 - ENSP00000349969 [99%]
SYNE2-202 - ENSP00000350719 [99%]
  SYNE2-001 - ENSP00000341781 [100%]
SYNE2-002 - ENSP00000452570 [100%]
SYNE2-011 - ENSP00000450831 [100%]
SYNE2-014 - ENSP00000378249 [100%]
SYNE2-201 - ENSP00000349969 [100%]
SYNE2-202 - ENSP00000350719 [100%]
 

Other gene match

    
ANTIGEN VIEW
SYNE2-001
SYNE2-002
SYNE2-011
SYNE2-014
SYNE2-201
SYNE2-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA003435

  

Antibody HPA050204

  

1.

 Super-resolution microscopy reveals LINC complex recruitment at nuclear indentation sites
Versaevel M et al
Sci Rep 2014;4:7362
Application: IHC 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.