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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 20 cell types
RNA: detected in 36 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:800
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047171
Antibody CAB009533
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
64.7, 29.9
64.7, 58.1, 57.2, 29.9
Antibody dilution
1:400
1:500
PROTEIN ARRAY
Antibody HPA047171
Antibody CAB009533
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
