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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:22
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA026307
Antibody HPA051783
Antibody HPA051785
Antibody HPA053973
Antibody CAB006849
Standard validation
Supported
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate positivity in subsets of neuronal cells.
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 21 tissues Protein: detected in 12 cell types
RNA: detected in 21 tissues Protein: detected in 47 cell types
RNA: detected in 21 tissues Protein: detected in 46 cell types
RNA: detected in 21 tissues Protein: detected in 42 cell types
RNA: detected in 21 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:275
1:10
1:175
1:300
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Standard validation
Supported
Figure description
Cell bodies and dendrites were stained in subsets of neurons, most abundant in olfactory bulb, cerebral cortex and cerebellum.
WESTERN BLOT
Antibody HPA026307
Antibody HPA051783
Antibody HPA051785
Antibody HPA053973
Antibody CAB006849
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
