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ACAA1

ANTIBODY INFORMATION
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006764   HPA007244  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   3  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA006764

  

Antibody HPA007244

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA007244.		  
Validated

Antibody staining overlaps with antibody HPA006764.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.   Immunofluorescent staining of human cell line U-251 MG shows localization to peroxisomes.  

Antibody dilution

 1:5   1:91  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.3, 40.3, 30.2   44.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA006764

  

Antibody HPA007244

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 56   128  

Antigen sequence

 
GNSSQVSDGAAAILLARRSKAEELGLPILGVLRSYAVVGVPPDIMGIGPA
YAIPVA
  
VLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQFLSDIPETVPLSTVNR
QCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLADRGNPGNITSRLMEK
EKARDCLIPMGITSENVAERFGISREKQ
 

Matching transcripts

 ACAA1-001 - ENSP00000333664 [100%]
ACAA1-007 - ENSP00000395183 [100%]
ACAA1-014 - ENSP00000398172 [100%]
  ACAA1-001 - ENSP00000333664 [100%]
 

Other gene match

    
ANTIGEN VIEW
ACAA1-001
ACAA1-007
ACAA1-014

RELEVANT PUBLICATIONS
 
 

Antibody HPA006764

  

Antibody HPA007244

  

1.

   Peripheral nervous system plasmalogens regulate Schwann cell differentiation and myelination
da Silva TF et al
J Clin Invest 2014;124(6):2560-70
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.