Fields »
ABOUT
HELP
BLOG
HAGH

ANTIBODY INFORMATION
 
 

Antibody HPA043041

  

Antibody HPA061143

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043041   HPA061143  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA043041

  

Antibody HPA061143

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043041

  

Antibody HPA061143

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:850  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043041

  

Antibody HPA061143

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.8, 28.9, 27.9, 26.3, 17.3   33.8, 28.9, 27.9, 26.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043041

  

Antibody HPA061143

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA043041

  

Antibody HPA061143

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   57  

Antigen sequence

 
NEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTS
GHICYFVSKPGGSEPPAVFTGDTLFVAGC
  
CGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGN
AAIREKL
 

Matching transcripts

 HAGH-001 - ENSP00000380514 [100%]
HAGH-002 - ENSP00000380511 [100%]
HAGH-003 - ENSP00000455422 [100%]
HAGH-010 - ENSP00000455970 [100%]
HAGH-012 - ENSP00000455355 [100%]
  HAGH-001 - ENSP00000380514 [100%]
HAGH-002 - ENSP00000380511 [100%]
HAGH-003 - ENSP00000455422 [100%]
HAGH-012 - ENSP00000455355 [100%]
 

Other gene match

    
ANTIGEN VIEW
HAGH-001
HAGH-002
HAGH-003
HAGH-010
HAGH-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.