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ANTIBODY INFORMATION
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015825   HPA050257  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA015825

  

Antibody HPA050257

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050257.		  
Validated

Antibody staining overlaps with antibody HPA015825.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:143   1:43  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human rectum shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:450    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 402.2, 43   402.2  

Antibody dilution

 1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA015825

  

Antibody HPA050257

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 146   134  

Antigen sequence

 
NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRS
RSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQ
HSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI
  
GEAVENQEVQSKKPIPSKPQLKQLQVLDDQGPEREDVRKNYCSLRDETPE
RKSGQEKSHSVNTEEKIGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKF
GSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSK
 

Matching transcripts

 SPEN-001 - ENSP00000364912 [100%]
SPEN-002 - ENSP00000388021 [100%]
  SPEN-001 - ENSP00000364912 [99%]
 

Other gene match

    
ANTIGEN VIEW
SPEN-001
SPEN-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.