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COL17A1

ANTIBODY INFORMATION
 
 

Antibody HPA043673

  

Antibody HPA052963

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043673   HPA052963  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   13  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA043673

  

Antibody HPA052963

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043673

  

Antibody HPA052963

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human skin shows strong positivity in keratinocytes.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 20 cell types		   RNA: detected in 23 tissues
Protein: detected in 44 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:15  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043673

  

Antibody HPA052963

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 150.4, 142.3, 44.1   150.4, 142.3, 44.1  

Antibody dilution

 1:250   1:160  

PROTEIN ARRAY
 
 

Antibody HPA043673

  

Antibody HPA052963

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:1250  

ANTIGEN INFORMATION
 
 

Antibody HPA043673

  

Antibody HPA052963

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 97   90  

Antigen sequence

 
DGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTH
GSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTR
  
NLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMA
SCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTTTSTA
 

Matching transcripts

 COL17A1-001 - ENSP00000340937 [100%]
COL17A1-003 - ENSP00000358748 [100%]
COL17A1-007 - ENSP00000376905 [100%]
  COL17A1-001 - ENSP00000340937 [100%]
COL17A1-003 - ENSP00000358748 [100%]
COL17A1-007 - ENSP00000376905 [100%]
 

Other gene match

    
ANTIGEN VIEW
COL17A1-001
COL17A1-003
COL17A1-007

RELEVANT PUBLICATIONS
 
 

Antibody HPA043673

  

Antibody HPA052963

  

1.

 Dual roles of hemidesmosomal proteins in the pancreatic epithelium: the phosphoinositide 3-kinase decides
Laval S et al
Oncogene 2014;33(15):1934-44
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.