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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Antibody dilution
1:52
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA056557
Antibody CAB033426
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes and in sebaceous glands.
Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in keratinocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
RNA: detected in 37 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:30
1:15
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056557
Antibody CAB033426
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.7, 29.5
32.7, 29.5
Antibody dilution
1:170
1:500
PROTEIN ARRAY
Antibody HPA056557
Antibody CAB033426
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
