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SPI1

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GENERAL INFORMATION

Gene name

 SPI1

Gene description

 Spi-1 proto-oncogene

Protein class

 Predicted intracellular proteins
Predicted membrane proteins
Transcription factors

Predicted localization

 Intracellular,Membrane

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the nucleoplasm.

RNA cell category

 Cell line enhanced (HMC-1, NB-4, THP-1, HL-60)

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA044653
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ASSAYS
RNA expression
Human cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Cell line enhanced (HMC-1, NB-4, THP-1, HL-60)
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the nucleoplasm.

Main location

 Nucleoplasm (supported)

 

 

 
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Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA044653 REH Nucleoplasm Intensity

GENE INFORMATION

Gene name

 SPI1 (HGNC Symbol)

Synonyms

 OF, PU.1, SFPI1, SPI-1, SPI-A

Description

 Spi-1 proto-oncogene (HGNC Symbol)

Entrez gene summary

 This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Chromosome

 11

Cytoband

 p11.2

Chromosome location (bp)

 47354860 - 47378576

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000066336 (version 83.38)

Entrez gene

 6688

UniProt

 P17947 (UniProt - Evidence at protein level)

neXtProt

 NX_P17947

Antibodypedia

 SPI1 antibodies

PROTEIN BROWSER
SPI1-001
SPI1-002
SPI1-003
SPI1-004

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
SPI1-001
ENSP00000367799
ENST00000378538
P17947 [Direct mapping]
Transcription factor PU.1
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Predicted intracellular proteins
Transcription factors
   Helix-turn-helix domains
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000790 [nuclear chromatin]
GO:0000980 [RNA polymerase II distal enhancer sequence-specific DNA binding]
GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding]
GO:0001047 [core promoter binding]
GO:0001085 [RNA polymerase II transcription factor binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0003705 [transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding]
GO:0003723 [RNA binding]
GO:0005515 [protein binding]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0030154 [cell differentiation]
GO:0043565 [sequence-specific DNA binding]
GO:0043966 [histone H3 acetylation]
GO:0044027 [hypermethylation of CpG island]
GO:0045646 [regulation of erythrocyte differentiation]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0051525 [NFAT protein binding]
GO:0090241 [negative regulation of histone H4 acetylation]
GO:1902895 [positive regulation of pri-miRNA transcription from RNA polymerase II promoter]
Show all
 270 aa
31.1 kDa 		No 0
SPI1-002
ENSP00000438846
ENST00000533968
F5H3K6 [Direct mapping]
Transcription factor PU.1
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Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0016021 [integral component of membrane]
GO:0030154 [cell differentiation]
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 245 aa
27.4 kDa 		No 1
SPI1-003
ENSP00000443865
ENST00000533030
F5GZ94 [Direct mapping]
Transcription factor PU.1
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0030154 [cell differentiation]
Show all
 35 aa
3.7 kDa 		No 0
SPI1-004
ENSP00000227163
ENST00000227163
P17947 [Direct mapping]
Transcription factor PU.1
Show all
Predicted intracellular proteins
Transcription factors
   Helix-turn-helix domains
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0000790 [nuclear chromatin]
GO:0000978 [RNA polymerase II core promoter proximal region sequence-specific DNA binding]
GO:0000979 [RNA polymerase II core promoter sequence-specific DNA binding]
GO:0000980 [RNA polymerase II distal enhancer sequence-specific DNA binding]
GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding]
GO:0000982 [transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding]
GO:0001047 [core promoter binding]
GO:0001077 [transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding]
GO:0001078 [transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding]
GO:0001085 [RNA polymerase II transcription factor binding]
GO:0001205 [transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding]
GO:0001944 [vasculature development]
GO:0002320 [lymphoid progenitor cell differentiation]
GO:0002573 [myeloid leukocyte differentiation]
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0003705 [transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding]
GO:0003723 [RNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006366 [transcription from RNA polymerase II promoter]
GO:0030098 [lymphocyte differentiation]
GO:0030154 [cell differentiation]
GO:0030218 [erythrocyte differentiation]
GO:0030225 [macrophage differentiation]
GO:0030851 [granulocyte differentiation]
GO:0035019 [somatic stem cell population maintenance]
GO:0043011 [myeloid dendritic cell differentiation]
GO:0043565 [sequence-specific DNA binding]
GO:0043966 [histone H3 acetylation]
GO:0044027 [hypermethylation of CpG island]
GO:0045347 [negative regulation of MHC class II biosynthetic process]
GO:0045646 [regulation of erythrocyte differentiation]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0051525 [NFAT protein binding]
GO:0060033 [anatomical structure regression]
GO:0071361 [cellular response to ethanol]
GO:0090241 [negative regulation of histone H4 acetylation]
GO:1902262 [apoptotic process involved in patterning of blood vessels]
GO:1902895 [positive regulation of pri-miRNA transcription from RNA polymerase II promoter]
Show all
 271 aa
31.2 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.