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ARFGEF1

ANTIBODY INFORMATION
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023399   HPA023822  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA023399

  

Antibody HPA023822

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA023822.		  
Validated

Antibody staining overlaps with antibody HPA023399.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.  

Antibody dilution

 1:100   1:20  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:30  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

   Approved  

   

Figure description

   Subsets of neurons showed strong staining in cell bodies and dendrites.  

WESTERN BLOT
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 208.8   208.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:10000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA023399

  

Antibody HPA023822

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 104   87  

Antigen sequence

 
VLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTI
EDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSP
GAKF
  
QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEH
EGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEA
 

Matching transcripts

 ARFGEF1-001 - ENSP00000262215 [100%]
  ARFGEF1-001 - ENSP00000262215 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARFGEF1-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA023399

  

Antibody HPA023822

  

1.

 The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2
Christis C et al
J Cell Biol 2012;196(3):327-35
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.