Fields »
ABOUT
HELP
BLOG
PLPP1

ANTIBODY INFORMATION
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA047815   sc-51394  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047815

  

Antibody CAB033331

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated
    
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.  

Antibody dilution

   1:6  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human prostate shows strong membranous and cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 24 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1500    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 32.2, 32.1   32.2, 32.1  

Antibody dilution

 1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:4950    

ANTIGEN INFORMATION
 
 

Antibody HPA047815

  

Antibody CAB033331

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 37    

Antigen sequence

 
DFFKERTSFKERKEEDSHTTLHETPTTGNHYPSNHQP
   

Matching transcripts

 PLPP1-001 - ENSP00000264775 [100%]
PLPP1-002 - ENSP00000302229 [100%]
   

Other gene match

    
ANTIGEN VIEW
PLPP1-001
PLPP1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.