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DNTTIP2

ANTIBODY INFORMATION
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044502   HPA053707  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044502

  

Antibody HPA053707

  
Immunocytochemistry


Validated
    
Immunohistochemistry


Validated
  

Validated
  
Western blot


Validated
   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:156    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.    

Antibody dilution

 1:156    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA053707 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA044502 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA053707 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA044502 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows moderate nucleolar positivity in subset of cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows moderate cytoplasmic and nucleolar positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 37 cell types		   RNA: detected in 36 tissues
Protein: detected in 30 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1400   1:5000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 84.5    

Loading control

 Total protein image    

Antibody dilution

 1:349    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 84.5   84.5  

Antibody dilution

 1:250   1:1750  

PROTEIN ARRAY
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:17400  

ANTIGEN INFORMATION
 
 

Antibody HPA044502

  

Antibody HPA053707

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   92  

Antigen sequence

 
PSQESHTEAISDAETSSSDISFSGIATRRTRSMQRKLKAQTEKKDSKIVP
GNEKQIVGTPVNSEDSDTRQTSHLQARSLSEINKPNFYNNDFDDDFSHR
  
DEGKEINEKSSQLKNLSELQDTSLQQLVSQRHSTPQNKNAVSVHSNLNSE
AVMKSLTQTFATVEVGRWNNNKKSPIKASDLTKFGDCGGSDD
 

Matching transcripts

 DNTTIP2-001 - ENSP00000411010 [100%]
  DNTTIP2-001 - ENSP00000411010 [100%]
 

Other gene match

    
ANTIGEN VIEW
DNTTIP2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.