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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Antibody dilution
1:81
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA067204
Antibody CAB004231
Standard validation
Supported
Figure description
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.
Expression
RNA: detected in 29 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067204
Antibody CAB004231
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
88.2, 87.7, 85.2, 75.7, 39.2
88.2, 87.7, 85.2, 75.7, 39.2, 12.5
Antibody dilution
1:180
1:500
PROTEIN ARRAY
Antibody HPA067204
Antibody CAB004231
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
