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Antibody staining overlaps with antibody HPA023273. Antibody staining overlaps with antibody HPA022912.
Validated
Antibody staining overlaps with antibody HPA023273. Antibody staining overlaps with antibody HPA022875.
Validated
Antibody staining overlaps with antibody HPA022912. Antibody staining overlaps with antibody HPA022875.
Standard validation
Approved
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Antibody dilution
1:200
1:10
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA022875
Antibody HPA022912
Antibody HPA023273
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:10
1:300
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA022875
Antibody HPA022912
Antibody HPA023273
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403068)
Target mass (kDa)
65.3, 62.3
65.3, 62.3, 23.7, 16.1
65.3, 62.3, 12.9
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA022875
Antibody HPA022912
Antibody HPA023273
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
