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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:61
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002718
Standard validation
Approved
Figure description
Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes and extracellular positivity in blood vessels.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002718
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
36.1, 35.9, 21.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002718
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
