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ATP1B3

ANTIBODY INFORMATION
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA048963   3019.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA048963

  

Antibody CAB020697

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.    

Antibody dilution

 1:53    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human adrenal gland shows moderate cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 31.5, 15   31.5, 15, 13.5, 10  

Antibody dilution

 1:260   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2100    

ANTIGEN INFORMATION
 
 

Antibody HPA048963

  

Antibody CAB020697

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 58    

Antigen sequence

 
KPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISLLQACSGMNDPDFGY
SQGNPCIL
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP1B3-001 - ENSP00000286371 [100%] 
ATP1B3-004 - ENSP00000417522 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP1B3-001
ATP1B3-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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