AP3M2 - Antibodies - The Human Protein Atlas

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AP3M2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Provider

Atlas Antibodies
Sigma-Aldrich

SDIX

Product name

HPA045591

HPA048380

2107

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

AP3M1 - ENSG00000185009 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 62 cell types

RNA: detected in 37 tissues
Protein: detected in 36 cell types

RNA: detected in 37 tissues
Protein: detected in 69 cell types

Retrieval

HIER pH6

Antibody dilution

1:10

1:200

1:50

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

47, 46.9, 30.4, 16

47, 46.9, 31.8, 30.4

47, 31.8, 30.4, 16, 14.7, 10.6, 1.2

Antibody dilution

1:250

1:220

1:500

PROTEIN ARRAY

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1800

ANTIGEN INFORMATION

Antibody HPA045591

Antibody HPA048380

Antibody CAB014876

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

SGDIFLEKHWKSVVSQSVCDYFFEAQ

VKYTNNEAYFDVVEEIDAIIDKSGS

Matching transcripts

AP3M2-001 - ENSP00000380132 [96%]
AP3M2-002 - ENSP00000428787 [96%]
AP3M2-003 - ENSP00000429435 [96%]
AP3M2-007 - ENSP00000429515 [96%]
AP3M2-201 - ENSP00000174653 [96%]

AP3M2-001 - ENSP00000380132 [96%]
AP3M2-002 - ENSP00000428787 [96%]
AP3M2-003 - ENSP00000429435 [96%]
AP3M2-006 - ENSP00000430616 [96%]
AP3M2-201 - ENSP00000174653 [96%]

Other gene match

AP3M1 - ENSG00000185009 [100%]

ANTIGEN VIEW

AP3M2-001	AP3M2-002	AP3M2-003	AP3M2-006	AP3M2-007	AP3M2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.