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CDC42

ANTIBODY INFORMATION
 
 

Antibody CAB004360

  

Provider

 Santa Cruz Biotechnology
  

Product name

 sc-8401  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Protein A/G  

Other gene match

  

Released in version

 2  

References

  

VALIDATION SUMMARY
 
 

Antibody CAB004360

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB004360

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.  

Antibody dilution

 1:25  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB004360

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB004360

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 21.3, 15.2  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB004360

  

Antigen

 Synthetic peptide  

Length (aa)

  

Antigen sequence

  

Matching transcripts

  

Other gene match

  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.