LIMS2 - Antibodies - The Human Protein Atlas

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LIMS2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA058340

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA058340

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA058340

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058340

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line BJ shows localization to focal adhesion sites.

Antibody dilution

1:72

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA058340

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 6 cell types

Retrieval

HIER pH6

Antibody dilution

1:700

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058340

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

41.5, 41.2, 38.9, 38.4, 28.4, 21.6, 18.9

Antibody dilution

1:240

PROTEIN ARRAY

Antibody HPA058340

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2900

ANTIGEN INFORMATION

Antibody HPA058340

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

KRLKKLSELTSRKAQPKATDLNSA

Matching transcripts

LIMS2-001 - ENSP00000347240 [100%]
LIMS2-002 - ENSP00000386637 [100%]
LIMS2-003 - ENSP00000387002 [100%]
LIMS2-004 - ENSP00000386383 [100%]
LIMS2-005 - ENSP00000326888 [100%]
LIMS2-006 - ENSP00000386570 [100%]
LIMS2-007 - ENSP00000397253 [100%]
LIMS2-008 - ENSP00000386345 [100%]
LIMS2-009 - ENSP00000386252 [100%]
LIMS2-012 - ENSP00000386907 [100%]
LIMS2-018 - ENSP00000443794 [100%]
LIMS2-201 - ENSP00000484949 [100%]

Other gene match

ANTIGEN VIEW

LIMS2-001	LIMS2-002	LIMS2-003	LIMS2-004	LIMS2-005	LIMS2-006	LIMS2-007	LIMS2-008	LIMS2-009	LIMS2-012	LIMS2-018	LIMS2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.