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LMAN1

ANTIBODY INFORMATION
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA002320   E1031  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 3   12  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA002320

  

Antibody CAB037163

  
Immunocytochemistry

Uncertain    
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.    

Antibody dilution

 1:24    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with granular pattern in glandular cells.   Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:900  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 57.5   57.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA002320

  

Antibody CAB037163

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 107    

Antigen sequence

 
IGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQNTLTVMIN
NGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQL
TEPGKEP
  

											 
																				

																		

										
											
Matching transcripts

												
																					LMAN1-001 - ENSP00000251047 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LMAN1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002320

												
										 
																				
											
											
											
Antibody CAB037163

												
										 
																		

																	

										
											
1.

												
																						High frequency of LMAN1 abnormalities in colorectal tumors with microsatellite instability

	Roeckel N et al

	Cancer Res 2009;69(1):292-9

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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