ACAT1 - Antibodies - The Human Protein Atlas

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ACAT1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA004428

Antibody HPA007569

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA004428

HPA007569

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA004428

Antibody HPA007569

Immunocytochemistry

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA004428

Antibody HPA007569

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA007569.

Validated

Antibody staining overlaps with antibody HPA004428.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Antibody dilution

1:25

1:222

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA004428

Antibody HPA007569

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA007569 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA004428 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 77 cell types

RNA: detected in 37 tissues
Protein: detected in 60 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:750

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA004428

Antibody HPA007569

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

45.2, 14.1

45.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA004428

Antibody HPA007569

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA004428

Antibody HPA007569

Antigen

Recombinant protein fragment

Length (aa)

142

128

Antigen sequence

VSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNV
LQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGH
QDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTD

NEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKR
VDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTP
LARIVAFADAAVEPIDFPIAPVYAASMV

Matching transcripts

ACAT1-001 - ENSP00000265838 [100%]
ACAT1-009 - ENSP00000436096 [85%]

ACAT1-001 - ENSP00000265838 [100%]

Other gene match

ANTIGEN VIEW

ACAT1-001	ACAT1-009

RELEVANT PUBLICATIONS

Antibody HPA004428

Antibody HPA007569

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51

Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism: implications for breast cancer prevention
Sanchez-Alvarez R et al
Cell Cycle 2013;12(2):289-301

Ketone body utilization drives tumor growth and metastasis
Martinez-Outschoorn UE et al
Cell Cycle 2012;11(21):3964-71

Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy
Chang HT et al
Nutr Metab (Lond) 2013;10(1):47

Ketone bodies and two-compartment tumor metabolism: stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells
Martinez-Outschoorn UE et al
Cell Cycle 2012;11(21):3956-63

Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection
Andersson S et al
J Histochem Cytochem 2013;61(11):773-84

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.