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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm and GFP expression in cytoplasm.
Antibody dilution
1:42
Standard validation
Uncertain
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:42
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043255
Antibody HPA043639
Antibody CAB046468
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows positivity in cells in seminiferus ducts.
Expression
RNA: detected in 14 tissues Protein: detected in 57 cell types
RNA: detected in 14 tissues Protein: detected in 60 cell types
RNA: detected in 14 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:125
1:500
1:4000
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043255
Antibody HPA043639
Antibody CAB046468
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
166.5, 166
166.5, 166
166.5, 166, 34.7
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA043255
Antibody HPA043639
Antibody CAB046468
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.