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ACTN2

ANTIBODY INFORMATION
 
 

Antibody HPA006035

  

Antibody HPA008315

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006035   HPA008315  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ACTN1 - ENSG00000072110 [100%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]
    

Released in version

 3   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA006035

  

Antibody HPA008315

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006035

  

Antibody HPA008315

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 10 tissues
Protein: detected in 74 cell types		   RNA: detected in 10 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:1800  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006035

  

Antibody HPA008315

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 107.6, 107.1, 105.6, 104.9, 103.9, 103.2, 103.1, 102.7, 94.8, 29.6, 27.1   103.9, 79.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA006035

  

Antibody HPA008315

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA006035

  

Antibody HPA008315

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   142  

Antigen sequence

 
SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYG
KLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSS
FYHAFSGAQKAETAANRICKV
  
AHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDE
LRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNK
MEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGD
 

Matching transcripts

 ACTN2-201 - ENSP00000443495 [83%]
ACTN2-001 - ENSP00000355537 [82%]
  ACTN2-001 - ENSP00000355537 [100%]
ACTN2-201 - ENSP00000443495 [100%]
ACTN2-202 - ENSP00000438384 [100%]
 

Other gene match

 ACTN1 - ENSG00000072110 [100%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]
    
ANTIGEN VIEW
ACTN2-001
ACTN2-201
ACTN2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.